The invention discloses a process for preparing a biomedical beta-titanium alloy. The process comprises the following steps: A) alloy preparation; B) surface coating; C) hot forging and cogging; D) hot rolling; E) cold rolling and molding; and F) heat treatment. In the process, the material is manufactured to a 2mm plate after the beta-titanium alloy is subjected to high vacuum electric arc melting, repeated stirring, casting, phase transformation point measuring, homogenization under the protection of vacuum and argon, coating, hot forging, hot rolling, cold rolling, solid solution, water quenching, artificial aging and water cooling so as to prepare the biomedical beta-titanium alloy with excellent comprehensive performance. The process is a biomedical beta-titanium preparation process capable of improving the material strength, abrasion resistance and corrosion resistance, reducing the elasticity modulus of the material, and maintaining excellent manufacturing and molding performance of the material.