The invention aims at providing bacteria for producing organic solvent resisting lipase, the organic solvent resisting lipase produced from the bacteria and the application of the organic solvent resisting lipase to the enzymatic reaction for the synthesis of the biodiesel in the organic phase, in particular to the ester exchange reaction for the synthesis of the biodiesel. The bacteria of pseudomonas aeruginosa LX1 is obtained through screening, the organic solvent resisting LX1 lipase produced from the pseudomonas aeruginosa LX1 has the amino acid sequence showed in the sequence identifier (SEQ ID) No. 2, and the organic solvent resisting LX1 lipase encoding gene has the nucleotide sequence showed in the SEQ ID No. 2. The organic solvent resisting LX1 lipase is used for catalyzing the substrate of soybean oil and exchanging the soybean oil and the ester methanol in the organic solvent tert-butanol system or the solvent-free system to synthesize the clean energy of biodiesel which has the conversion rate of 80-90 percent.