The invention belongs to the technical field of preparation of alkaline battery separators, and discloses novel electron beam irradiation grafting preparation technology for polyolefin non-woven fabric alkaline battery separators. The preparation technology comprises the steps of: taking a polyolefin non-woven fabric as a substrate, and making the polyolefin non-woven fabric absorbed with aqueoussolution of olefin monomers move in an unfolding state to perform electron beam irradiation through an electron beam irradiation device. The preparation technology has simple and continuous operationflow, can realize industrialized production, obtains few homopolymers, has high utilization rate of grafting monomers, prepares battery separators with uniform performance, and has lower cost, so thepreparation technology has extremely broad industrialized application prospect.