The invention discloses a device and a method for arranging a plurality of cells at the same plane and controlling the cells, comprising: a thin film with micro-hole arrays which is located at the upper surface of a base; a PDMS stamp whose lower surface is provided with a micro-groove unit and is coated on the thin film; the micro-groove unit comprises: an intermediate groove and a left-side groove and a right-side groove which are located at the left side and the right side of the intermediate groove; the middle sections of the left-side groove and the right-side groove are parallel to the intermediate groove; the both end sections except for the middle sections of the left-side groove and the right-side groove slant towards the direction away from the intermediate groove; the end of the groove is provided with a vertical through hole which is communicated with the corresponding groove; the holes of the thin film are all located in the micro-groove of the stamp; and a micro-pipelineis provided with different cell suspensions, the cell grows in the micro-pipeline and adheres to the micro-pipeline; the stamp is removed to realize the co-culture of a plurality of cells at the sameplane; and then the thin film is removed to realize the free movement of the plurality of co-cultured cells at the same plane; the method is simple to operate, the used material is cheap, and the invention can be used for high-throughput screening of useful drugs for cells.