The invention discloses a lithium ion battery cell packet preparation method, including the following steps: pole piece material is made into negative pole pieces, and the negative pole pieces are transmitted to a negative pole sorting machine; qualified negative pole pieces are selected from the negative pole pieces and transmitted to a lamination stacking machine; the pole piece material is made into positive poles pieces, and the positive pole pieces are transmitted to a positive pole sorting machine; qualified positive pole pieces are selected from the positive pole pieces and transmittedto a positive pole bag machine; the qualified positive pole pieces are made into positive pole bags, and the positive pole bags are transmitted to the lamination stacking machine; lamination stackingis carried out on the positive pole bags and the qualified negative pole pieces by the lamination stacking machine, thus obtaining the cell packet. The invention also discloses a lithium ion battery cell packet preparation system. The invention comprises a negative pole processing machine, a split pole sorting machine, a lamination stacking machine, a positive pole bag machine, a positive pole sorting machine and a positive pole processing machine which are arranged in sequence, transmission equipment is arranged between each two equipment, thus cell packet can be prepared without manual operation and reliability and stability of the cell packet are improved.