The invention relates to a method for measuring chlorophyll content of fresh tobacco leaf of flue-cured tobacco based on canopy multi-spectra, which is characterized by comprising the following steps: measuring the canopy spectra of the flue-cured tobacco from a resettling stage to a budding early stage by a multispectral radiometer; establishing vegetation indices; measuring the chlorophyll content of the middle part of the reciprocal fifth leaf of the corresponding plant by a colorimetric method in a laboratory; establishing a model for inverting the chlorophyll content of the leaf by the canopy spectra parameters by a statistical method; measuring the canopy spectra parameters of a sample to be measured; and inputting the canopy spectra parameters into the model, thereby realizing measuring of the chlorophyll content of the flue-cured tobacco leaf. Compared with the prior art, the invention has the following advantages of in-situ measurement, no need of sampling, no injury to tobacco plants and no need of chemical reagents; compared with a hyperspectral radiometer, the invention has larger cost advantage and is more suitable for agricultural production; measurement can be finished only by acquiring data of a 550nm channel and a 730nm channel; and the data processing method and process are simple.