The invention provides a 4,4 '-bis-arylation acetenyl azobenzol organic light-emitting material, a preparation method and application. 4,4 '-bi-iodine azobenzol is used as a raw material, and two-triphenylphosphine palladium dichloride/ iodide copper is used as a catalyst. In the role of 3-isopropyl amine, the 4,4 '-bi-iodine azobenzol and terminal aryne generate Sonogashira coupling reaction in diethylene oxide for 20-50 hours to obtain series of 4,4 '-bis-arylation acetenyl azobenzol compounds. The compounds can be used as the organic light-emitting material.