The invention relates to a preparation method of sea-buckthorn enzymes and a product thereof, in particular to a method for preparing sea-buckthorn enzymes from seabuckthorn fruit mud. The preparation method comprises the following steps: 1) juicing fresh sea-buckthorn, extracting sea-buckthorn seed oil and carrying out vacuum drying on remaining seabuckthorn fruit mud; 2) pulverizing and sieving dried seabuckthorn fruit mud to prepare seabuckthorn fruit mud powder; 3) mixing the seabuckthorn fruit mud powder and water according to the proportion of 1:10 and sterilizing the mixture at 121 DEG C; 4) culturing the mixture at a table contractor, and freezing drying the mixture, finally obtaining dry seabuckthorn fruit mud enzymes. The sea-buckthorn enzymes prepared by the method of the invention contain a large amount of secondary metabolites such as protein, saccharides, phenols and flavonoid. As the novel cosmetic additive on the market, the sea-buckthorn enzymes enjoy very promising application prospect.