The invention provides a light-emitting diode drive circuit with a temperature compensation function, which comprises a conversion circuit, a light-emitting diode module and a controller. The conversion circuit receives the power of an input voltage and converts the input voltage into an output voltage according to a control signal. The light-emitting diode module is coupled with the conversion circuit. According to a voltage feedback signal representing the output voltage and a control signal outputted by an operating temperature, the controller controls the output voltage to decrease as theoperating temperature increases. Consequently, the light-emitting diode drive circuit has the compensation effect of decreasing the drive voltage of the light-emitting diode module as the temperatureincreases.