The invention relates to aschersonia aleyrodis yellow non-woven cloth antibacterial and a preparation method thereof, i.e. a production process method for producing an aschersonia aleyrodis yellow non-woven cloth antibacterial of a fungal pesticide for effectively preventing sucking pests of orange gardens and tea gardens. The method comprises five production procedures including stain obtainment, one-stage bevel strain culturing, liquid seed culturing, liquid fermentation tank production and inoculation culturing of a yellow non-woven cloth bacteria strip. The pesticide has lasting control effect on the pests, obvious and stable effect on killing aschersonia pests with tendency to yellow habit in the orange gardens and tea gardens, little influence by weather, stable process, little investment, small land occupation space and no three wastes in production.