The invention relates to a combination of a sustained-release freshener used for disposable hygiene product, a preparation method and the application thereof. Raw materials of the combination are mainly are follows (proportion by weight of each component is shown in brackets): a freshening factor (10 to 30%), a sustained-release agent (30 to 50%), a preservative (0.1 to 1%), an inclusion agent (10 to 20%), an additive (1 to 25%) and a medical solvent (10 to 30%). With simple manufacturing process, cheap raw materials, good effect, strong adaptability and obvious economic as well as social benefits, the combination of the sustained-release freshener overcomes the stimulation to a human body due to high content of the freshening factor and improves the problem that the content utilizing rate of effective components of the common freshener is low.