The invention relates to moxifloxacin hydrochloride glucose injection and the preparation method thereof. The preparation method comprises the following steps: A. adding injection water into the ingredient tank and a proper amount of auxiliary material and ibuprofen, B. stirring and adjusting the PH value with base regulator to 6.5 - 9.0. Adding injection water to the needed amount and stirring by activated carbon pole for needle injection, decarbonising the titanium stick and refine the solution by millipore filter. C. conducting the encapsulation after the pass of the test. D. delivering the encapsulated semi-product into the sterilization case for autoclave sterilization.