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发明公开
CN101787371A 化学合成的HSV2病毒gD糖蛋白胞外区基因片段及其表达、应用 无效 - 撤回

化学合成的HSV2病毒gD糖蛋白胞外区基因片段及其表达、应用
摘要：
本发明化学合成的HSV2病毒gD糖蛋白胞外区基因片段及其表达、应用涉及基因工程技术、疫苗和诊断试剂领域。本发明通过计算机分析，筛选出含强抗原表位的HSV2病毒gD糖蛋白胞外区片段，第1个氨基酸至第286个氨基酸，共286个氨基酸，选择真核和原核生物均偏爱的密码子，化学合成抗原表位的全新基因序列，利用基因工程技术表达该基因片段、制备HSV2病毒gD糖蛋白的强抗原表位片段及HSV2病毒gD糖蛋白的抗血清。表达的HSV2病毒gD糖蛋白可用于疫苗、HSV2病毒抗体或抗原的检测及用于免疫制备抗HSV2病毒单抗和多抗等。
摘要（英）：

The invention discloses a chemosynthetic HSV2 virus gD glycoprotein extracellular region gene segment as well as expression and application thereof, which relates to the technical field of gene engineering and the fields of vaccines and diagnosing agents. In the invention, through computer analysis, an HSV2 virus gD glycoprotein extracellular region segment containing strong antigen epitope is screened, and the HSV2 virus gD glycoprotein extracellular region segment contains 286 amino acid from the 1st amino acid to the 286th amino acid, and codons favored by both eucaryon and procaryote are selected to chemically synthesize a new gene sequence with the strong antigen epitope. Therefore, the gene segment is expressed by using the gene engineering technology, and the strong antigen epitope segment of HSV2 virus gD glycoprotein and antiserum of the HSV2 virus gD glycoprotein are prepared. The expressed HSV2 virus gD glycoprotein can be used for detecting vaccines, HSV2 virus antibody or antigen and preparing anti-HSV2 virus mono-antibody, poly-antibody and the like for immunity.

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