The invention provides the use of cis-butenedioic anhydride compounds in the preparation of Sclerotinia sclerotiorum bacteriostats. In the invention, the high fungus inhibition performance and the characteristics of simple synthesis, cheap raw material, small toxic and side effect on human bodies and the like are fully used, and the cis-butenedioic anhydride compounds having high Sclerotinia sclerotiorum inhibition effect are screen out. As the cis-butenedioic anhydride compounds has the characteristics of cheap synthesis raw materials, simple and mature synthesis method, small toxic and side effect on human bodies and high Sclerotinia sclerotiorum inhibition effect, novel sclerotiniose resistance medicaments of high application values are expected to be further developed.