The invention discloses a method for measuring sulfur content in iron ore. The method comprises the following steps: dissolving a sample in aqua regia by heating or using microwave to obtain the solution to be measured; measuring the spectral intensity of three or more types of ferrum-based solution of which the known sulfur content ranges comprise the sulfur content of the sample by using ICP-OES; establishing a calibration curve; measuring the spectral intensity of the solution to be measured; and performing regression calculation according to the calibration curve to obtain the sulfur content in the solution to be measured. Because the ICP-OES has the characteristics of wide linear detection range, high sensitivity, and accuracy, by using the method, the sulfur content over 0.0005 percent in the iron ore can be measured. Simultaneously, when the calibration curve is established, a matrix effect of an iron matrix can be eliminated by performing matrix matching, so that the measurement result is more accurate and reliable. In addition, in the method, the sample is dissolved by heating or using microwave, so that the method is more scientific and accurate. Moreover, the method canbe realized only by simply dissolving the sample, measuring, establishing the calibration curve, and performing the regression calculation by using a computer, so the method has very high practicability.