The invention discloses a method for establishing an airway-free high-reactivity eosinophilic granulocyte airway inflammatory mouse model. The method comprises the following steps of: 1. preparation of a mouse: preparing a SPF (Specific Pathogen Free) level female Balb/c mouse with 5-6 week-ages; 2. molding of the mouse: respectively injecting 200mul of physiological saline solution mixed suspension of 10mug of chicken ovalbumin and 1.3mg of aluminum hydroxide into the abdominal cavity of the mouse at the 0th, the 7th and the 14th days of an experiment for carrying out sensitization; carrying out light anaesthesia on the mouse at the 21st, the 22nd and the 23rd days of the experiment, and carrying out nasal dripping excitation through 50mul of chicken ovalbumin solution with the mass percent concentration of 0.02 percent; and 3. treatment of the mouse: carrying out wounded lung function detection and bronchoalveolar lavage fluid cellular classification counting analysis on the mouse after the excitation of the last time for 24-48 hours, collecting the lung of the mouse to observe the histological change of the lung to obtain the hominine airway-free high-reactivity eosinophilic granulocyte airway inflammatory mouse model.