The invention relates to a repeated module gene-splicing method, which includes the following steps: (1) two PCR primers are utilized to amplify target gene; (2) the PCR primers and plasmid pET-LIC are cut by double restriction enzymes, segments are connected with carrier, and after competent cells are transformed, plasmid pET-TS is obtained; (3) the plasmid pET-TS is cut by double restriction enzymes, and plasmid segments are recovered, and are then connected with the target gene segments to transform, so that plasmid pET-TS2 is obtained; (4) the plasmid pET-TS2 is cut by double restriction enzymes, and plasmid segments are recovered, and are then connected with the target gene segments to transform, so that plasmid pET-TS3 is obtained; (5) the plasmid and target gene PCR product are cutby double restriction enzymes, and the same method is utilized to continuously insert repeated segments. The invention repetitively splices a plurality of repeated module genes, moreover, no restriction enzyme cutting site residues are left between the modules, thereby the seamless connection between the repeated modules is truly realized, and moreover, splicing is directional.