The invention discloses a seed production method for producing two-line hybrid rape by using photoperiod-temperature sensitive genic male sterile rape in a winter rape area, comprising the following steps of: preparing hybrids in the winter rape area; utilizing chemical anthericide treatment as an assistant measure of a photoperiod-temperature sensitive genic male sterile line of a female parent to prevent fertility fluctuation; and then hybridizing the female parent with a make parent to product the hybrids. The method has the advantages of expanding the seed production area of the rape and improving the fertility potential of the two-line hybrid rape.