The invention discloses a mixture of pine pollen/flaxseed powder/giant salamander powder and a preparation method thereof. The mixture comprises 10-30% of the pine pollen fine powder, 10-30% of the flaxseed fine powder and 40-80% of the giant salamander powder by weight percent, wherein the pine pollen, flaxseed powder and giant salamander powder are nano-grade fine powder. The preparation methodcomprises the following steps: performing wall-breaking treatment on the pine pollen and the flaxseed powder, and then grinding the powder into nano-grade fine powder; removing lungs and intestinal tract organs of live giant salamanders, freezing in vacuum, drying, pulverizing and then grinding the giant salamander into nano-grade fine powder; and finally mixing the three types of powder proportionally to obtain the product. The mixture has rich vitamin, unsaturated fatty acid and amino acid, has abundant nutrients and reasonable allocation, conforms to the requirements of food pyramid, has obvious food therapeutic effects on frequently-occurring diseases and common diseases such as hypertension, hyperlipaemia, hyperglycemia, arthritis, gastrointestinal diseases and the like, and has preventive effects on heart disease, apoplexia, cancers and the like; and the mixture is a nutrient health care product with convenient administration.