The invention discloses a mixture of black fungi, royal jelly and giant salamander full powder and a preparation method thereof. The black fungi, the royal jelly and the giant salamander full powder in the mixture are nanometer fine powder, and the black fungi fine powder accounts for 10 to 30 percent, the royal jelly fine powder accounts for 10 to 30 percent and the giant salamander full powder accounts for 10 to 80 percent in percentage by weight. The preparation method comprises the following steps of: after being sterilized, the black fungi are ground into nanometer fine powder; after being processed by vacuum freeze drying, the royal jelly is ground into nanometer fine powder; after lungs and intestinal tract organs are removed, live giant salamander is processed by vacuum freezing, drying and pulverization and then ground into nanometer fine powder; and the thee kinds of powders are proportionally mixed to prepare the product. The mixture is rich in vitamin, protein, iron and other microelements as well as unsaturated fatty acid and amino acid, has comprehensive nutrition ingredients and reasonable proportion, meets the requirements of the food pyramid and is the nutrient health care product capable of reducing blood-fat and blood viscosity, reducing blood sugar in an auxiliary manner, balancing fat metabolism, glycometabolism, and the like and being convenient to take.