The invention relates to a culture method of a new golden-yellow strain of Chlamys nobilis rich in natural carotenoid, which comprises the steps of: seed selection, single individual induced spawning, family establishment by monogam insemination, offspring seed culture, intermediate breeding and cultivating, color purification check, new strain multiplication and continuous selective breeding and the like; and the new golden-yellow strain of the Chlamys nobilis rich in natural carotenoid can be cultured by two-generation directional selective breeding. The new strain has stable character, notonly has golden-yellow shell, adductor muscle and pallium, but also is rich in the natural carotenoid; averagely, 98% of individuals have golden-yellow shells, 96% of individuals have golden-yellow adductor muscle, 99% of individuals have golden-yellow pallium, and the total content of the carotenoid is 4.4-13.7 times of that of groups not subjected to the selective breeding. The invention culture a new strain of shellfish from the angles of the sensed color and the nutrition; and the cultured new strain of Chlamys nobilis not only has beautiful color, but also is rich in the natural carotenoid, thereby achieving significant economic value, health care value and medical value and having broad market prospect.