The invention discloses a catalyst for catalyzing and reforming biologic oil to prepare hydrogen, comprising a catalyst active component and a catalyst carrier, wherein the catalyst carrier is CexZrl-xO2, x is 0.1-0.9, the catalyst active component is nickel, and the content of the catalyst active component is 5-20 percent of the weight of the catalyst carrier. The invention also provides a preparation method of the catalyst, comprising the following steps of: (1) dissolving soluble salts of nickel, cerium and zirconium into water, and the total concentration of the soluble salts is 0.05-1.5mol/l; and (2) adding an alkaline precipitant into the water solution, regulating a pH value of 8-12, separating precipitates, aging the precipitates, filtering to obtain a filter mass, washing, drying, roasting 2-6 hours at the temperature of 600-900 DEG C and obtaining the catalyst Ni-CexZrl-xO2. The prepared catalyst of the invention has higher catalytic activity and high hydrogen productive rate and is suitable for industrialized requirements.