The invention relates to an enzyme immobilization method by taking carbon fibers as novel carriers, in particular to an enzyme immobilized carrier needing a high-temperature reaction environment and an immobilization method thereof. The method comprises the following steps of: carrying out surface treatment on carbon fiber carriers by taking micron and nano-grade series carbon fibers with different diameters as the carriers; soaking and washing the degummed or gum-free carbon fiber carriers in absolute ethyl alcohol, and then naturally airing or drying; then balancing the carbon fibers with abuffer solution to obtain immobilized enzyme carriers; and immobilizing the surface-treated carbon fiber carriers as the carriers. The carriers are suitable for various catalyzing enzymes reacted in the high-temperature environment. The immobilized enzyme prepared by the invention has good stability in the high-temperature environment, is suitable for being applied to biochemical reaction and reactors and has important application value in the industry field.