The invention relates to a horizontal centrifuge with an isolation screen, which is mainly used for the solid-liquid separation of materials in the medicament manufacturing field, the food manufacturing field and highly-clean manufacturing field. The horizontal centrifuge with the isolation screen comprises a rotary drum, a base and a stand for supporting the base; the isolation screen is arranged between the base and the rotary drum and fixed on the stand and is provided with a round hole; a driving shaft of a driving mechanism which is arranged on the base penetrates the round hole of the isolation screen to be connected with the rotary drum; the driving mechanism is used for driving the rotary drum to rotate through the driving shaft; the rotary drum is provided with a housing; the base is provided with a round housing support plate, and the round housing support plate is arranged in the round hole of the isolation screen; the round housing is fixed on the round housing support plate; an unclean zone and a clean zone are arranged through the isolation screen; the base, the stand and the driving mechanism are arranged in the unclean zone; and the rotary drum, the housing of the rotary drum and the product contract part are arranged in the clean zone. Due to the arrangement of the isolation screen, the pollution to the clean zone by the base, the stand and the driving mechanism can be effectively prevented, so that the rotary drum and the like can be kept clean, the clean space area can be reduced, the production cost can be lowered, and the resources can be saved.