The invention discloses a method for detecting spiramycin and a special enzyme-linked immunoassay kit thereof. The enzyme-linked immunoassay kit comprises a spiramycin specific antibody, a coating and an enzyme marker, wherein the spiramycin specific antibody is a monoclonal or polyclonal antibody of spiramycin. The enzyme-linked immunoassay kit has the advantages of simple structure, convenient use, low price, portability, and high-efficiency, accurate, simple and convenient detection method, can be used for on-site monitoring, is suitable for qualitative and quantitative screening of a large number of samples, and is expected to play a significant role in the detection of spiramycin.