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发明公开
CN101845502A 一种多重连接与延伸依赖的探针扩增方法及其试剂盒 无效 - 驳回

一种多重连接与延伸依赖的探针扩增方法及其试剂盒
摘要：
本发明涉及一种多重连接与延伸依赖的探针扩增方法，所述的方法包括如下步骤：(1)DNA靶序列变性并与MLEPA探针进行杂交；(2)延伸与连接反应；(3)PCR反应；(4)PCR产物通过毛细管电泳分离；(5)电泳结果进行数据处理；所述的延伸与连接反应是同时使用DNA多聚酶与连接酶的延伸与连接反应。本发明还提供了一种能够同时扩增与检测多个DNA位点试剂盒。本发明的有益效果是：可以在一次反应中同时进行多个DNA位点的检测。由于其探针设计的灵活，MLEPA技术可被灵活广泛的应用到各类型STR位点与保守DNA位点的检测中。
摘要（英）：

The invention relates to a probe amplification method with multiple connection and extending independency, comprising the following steps that: (1) a DNA target sequence is denatured and is hybridized with an MLEPA probe; (2) extension and connection reaction is carried out; (3) PCR reaction is carried out; (4) PCR products are separated by capillary electrophoresis; (5) data processing is carried out on electrophoresis results; and the extension and connection reaction uses DNA polymerase and ligase simultaneously. The invention also provides a kit which can amplify and detect a plurality of DNA sites. The invention has the benefit of being capable of carrying out detection on the DNA sites simultaneously in primary reaction. Due to flexible design of the probe, the MLEPA technology can be flexibly and widely applied in detection of various STR sites and conservative DNA sites.

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