The invention discloses manufacturing method and equipment of a single optical frequency comb with high stability and high repetition frequency. The method comprises the following specific steps of: outputting femtosecond pulses with high repetition frequency, wide spectrum and pulse width less than 7 fs by using a cycle-grade femtosecond titanium sapphire oscillator; carrying out beat measurement of the CEO of the femtosecond pulses by using a single nonlinear crystal; and simultaneously locking repetition frequency and CEO frequency to a stable external microwave reference source by using a circuit feedback technology. The equipment comprises the cycle-grade femtosecond titanium sapphire oscillator, the single nonlinear crystal beat measurement fceo device and a feedback circuit device controlled on the basis of the phase lock principle. The invention has the characteristics of compact equipment structure, long locking time, high usable output power, small phase noise and the like, and is mainly applied to the scientific researches of absolute optical frequency measurement, optical clock composition, atto-second production and the like.