The invention provides a double-channel real-time bioaerosol monitoring method and a device thereof. The method is characterized by comprising the following steps: (1) concentrating atmospheric aerosol particles; (2) carrying out double-channel ultraviolet induced fluorescence detection; (3) data-processing; (4) cleaning the surface of an enrichment board of the particles and regenerating; and (5) repeatedly carrying out a new round of loop detection. The device comprises an aerosol-particles concentration unit, a double-channel ultraviolet induced fluorescence detection unit, a particle enrichment board regeneration mechanism, a rotary table and a control system. The invention can measure the density and the change of bio-particles such as bacteria, spores, viruses and the like in an ambient atmosphere in real time so as to achieve an early warning function on bioaerosol content in the atmosphere.