The invention relates to a method for measuring alpha acid and beta acid in hops by an ion pair chromatography, which is characterized by comprising the following steps: a, preparing samples: preparing a hops tinctures sample by weighing a given weight of hops tinctures, diluting the hops tinctures with four to six times of chromatographic pure methanol in proportion by weight and filtering the diluted hops tinctures with 0.25 mu m of an organic phase filter membrane; preparing hops extractum by weighing a given weight of extraxtum, dissolving the hops extractum with 380-420 times of chromatographic pure methanol in proportion by weight and filtering the dissolved hops extrantum with 0.25 mu m of the filter membrane; and b, analyzing the prepared samples in step a by a diode-array detector of a liquid chromatogram. Compared with an original method for analyzing alpha acid and beta acid in hops, the method uses ion pair agent to avoid using strongly acid mobile phase, realizes better separation effect for alpha acid and beta acid than that of the current method, has good repeatability and recovery rate of experiments and is suitable for analysis of large quantities of samples.