The invention provides refrigerated prepared instant sharkskin and a preparation method thereof, wherein the refrigerated prepared instant sharkskin has a raw material recipe consisting of sharkskin and bacteriostatic fresh-keeping liquid according to a ratio of 1/1 (w/v). The sharkskin is used as raw materials, deodorization and embitterment treatment is carried out on the sharkskin, a cold sterilization process is used for carrying out early stage sterilization treatment on the sharkskin after hot blanching curing and ice water sharp quenching, then, the bacteriostatic fresh-keeping liquid is added to the sharkskin for keeping the freshness, and the refrigerated prepared instant sharkskin is obtained through refrigeration after package. The production process of the refrigerated prepared instant sharkskin of the invention can effectively eliminate the fishy smell of the sharkskin raw materials, can improve the crispy mouth feeling of the refrigerated prepared instant sharkskin products, can prolong the fresh-keeping shelf life of the refrigerated prepared instant sharkskin, and can develop the refrigerated prepared instant sharkskin products with nutrition, good taste, unique features, sanitation and safety.