The invention discloses a real-time fluorescent RT-PCR method for detecting whether arabis mosaic virus, bean yellow mosaic virus and lily symptomless virus infect a host. In the invention, according to the RNAs of the arabis mosaic virus, the bean yellow mosaic virus and the lily symptomless virus, a specific amplimer and a fluorescent probe are designed, RT-Realt time PCR reaction is carried out the RNA template of virus to be identified by means of the specific amplimer and the fluorescent probe, and whether the arabis mosaic virus, the bean yellow the mosaic virus and the lily symptomless virus infect the host is rapidly detected by carrying out real-time fluorescent detection on RT-Real time PCR products.