The invention relates to an evaluation method of the ergonomics design of an ultrasonic probe. The method comprises the following steps of: demanding a user to simulate ultrasonic detection operation according to the posture and the movement of an ultrasonic doctor in ultrasonic detection; carrying out a specific operation test task by an operator by using ultrasonic probes with different shape designs or weights; recording surface myoelectric signals of a specific hand muscle of the operator by using a myoelectric signal recorder; preprocessing the acquired surface myoelectric signals; then,carrying out the statistical analysis of the amplitudes of the myoelectric signals through pairwise comparison; analyzing whether significant differences to a specific hand muscle load of the volunteer caused by the factors of the shape designs or the weights, and the like of the ultrasonic probes in test operation and using the significant differences as objective evaluation indexes of the ergonomics design of the ultrasonic probe; combining Borg scale subjective comfort score values corresponding to different contrast experiments; and evaluating the ergonomics design of the ultrasonic probe. The method overcomes the defects of a single evaluation index and can comprehensively evaluate the ultrasonic probe.