The invention discloses a recombinant vector for regulating the expression of two target genes by using a promoter, which has an improved pricking function in tumor cells. A construction method comprises the following steps of: 1, extracting a Survivin promoter gene; 2, extracting a cytogene mRNA of a human body, and performing reverse transcription on the cytogene mRNA to form cDNA; 3, amplifying a first target gene in the cDNA; 4, amplifying a second target gene in the cDNA; 5, connecting Survivin promoter gene segments cut by incision enzyme and basic vector molecules by using ligase to form a first intermediate vector, connecting first target gene segments cut by the incision enzyme and first intermediate vector molecules by using the ligase to form a second intermediate vector, connecting second target gene segments cut by the incision enzyme and second intermediate vector molecules by using the ligase to form a third intermediate vector, and connecting IRES gene segments cut by the incision enzyme and the third intermediate vector by using the ligase to form the recombinant vector; and 6, transfecting recipient cells with the recombinant vector, culturing the transfected cells, amplifying recombinant vector molecules with the recipient cells, screening the recipient cells containing the recombinant vector molecules, extracting the recombinant vector and detecting the tumor cell killing capability of the recombinant vector.