The invention discloses a manyflower tickclover herbal extract and application thereof, relating to Chinese herbal medicinal extracts and application thereof. A preparation method of the manyflower tickclover herbal extract comprises the steps of: taking roots, stems or leaves of the Chinese herbal medicinal manyflower tickclover herbs as raw materials, carrying out leaching by refluxing on the raw materials with 75-95% (V/V) of ethanol, and recovering the solvent ethanol to obtain ethanol total extractum; and dispersing the total extractum with water, extracting with ethyl acetate, and recovering an extracting agent to obtain an ethyl acetate extract. Tests prove that the extract has better effect on preventing and treating fatty liver diseases caused by hyperlipidemia and hyperlipidemiasyndrome and can be used for preparing medicaments for preventing and treating the fatty liver diseases caused by the hyperlipidemia and the hyperlipidemia syndrome; and compared with the traditionalmedicament, the medicament prepared from the invention has the advantages of low toxicity, safety, reliability, strong drug action, wide resources, simple process and low cost.