The invention relates to a three-dimensional barcode label and a manufacturing method thereof. The label consists of a unit matrix, which comprises positioning areas, an information coding area, an initial area and an ending area, wherein the positioning areas are positioned on two opposite borders of the unit matrix, and consist of positioning units, and two adjacent positioning units have different heights; the information coding area is positioned in the middle area of the unit matrix, and consists of information storage units composed of columns with different heights; and the other two opposite borders of the unit matrix are respectively the initial area and the ending area. The method for manufacturing the label comprises the following steps of: 1, converting a data character into a binary bit stream; 2, splitting the binary bit stream into a data segment and an error correcting code field; 3, splitting the data segment and the error correcting code field to form a code word stream of a code word; 4, compiling the code word stream into information storage units with different heights; 5, arranging the information into the matrix according to symbol requirement; 6, generating a three-dimensional barcode file by using a coder; and 7, manufacturing the three-dimensional barcode label by using a three-dimensional barcode printer.