The invention discloses a brain targeted liposome preparation of a <99m>Tc tumor imaging medicament and application thereof. In the invention, the idea that an active brain targeted liposome entraps the <99m>Tc tumor imaging medicament is put forward, and the brain targeted liposome preparation of the <99m>Tc tumor imaging medicament is prepared by a fast film dispersion method, wherein liposome has the entrapment rate of over 99 percent, the particle size of less than 30 nm and a structure shown in figure 1. Phospholipid is dispersed in water to form single-layer or multi-layer microcapsules; the liposoluble <99m>Tc medicament is inserted into a bimolecular lipid membrane; and a bradykinin analogue RMP-7 is spread on the surface of the liposome as an active brain targeted directing molecule. Mouse tissue distribution experiments show that the preparation greatly improves the capability of the <99m>Tc medicament in passing through BBB; the SPECT imaging and self-development results of a model of mice with C6 brain gliomas show that the active targeted liposome preparation can realize the early diagnosis of brain tumors when applied to the <99m>Tc tumor imaging medicament; and the brain targeted liposome preparation belongs to the technical field of radiopharmacy and medicinal preparations.