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发明公开
CN101921803A 一种耐盐碱基因去除标记重组质粒载体 无效 - 撤回

一种耐盐碱基因去除标记重组质粒载体
摘要：
一种耐盐碱基因去除标记重组质粒载体，属于植物转基因技术领域，是用SKC1及35S启动子替换了基础载体PX6-GFP的GFP序列而构建成的，这个基因位于载体T-DNA左边界LB和右边界RB序列之间，它含有的XVE杂合蛋白与诱导物17-β-雌二醇结合后可启动重组酶CRF的表达，从而剔除选择标记，启动子为人工启动子G10-35S，目的基因是耐盐性基因SKC1。本发明的载体转化率较高；并且本发明获得的转基因玉米具有较好的耐盐碱特性。
摘要（英）：

The invention relates to a salt and alkali resistance-associated gene mark-removing recombinant plasmid vector, which belongs to the field of plant transgenic technology. The salt and alkali resistance-associated gene mark-removing recombinant plasmid vector is constructed by substituting SK1 and 35S promoters for a GFP sequence of a basic vector PX6-GFP; the gene is between the left border (LB) sequence and the right border (RB) sequence of a vector T-DNA, the XVE hybrid protein of the gene can be combined with 17-beta-estradiol inducer to promote the expression of a recombinase CRF and thus, a selective mark is removed; and the promoter is an artificial promoter G10-35S, and the target gene is salt resistance-associated gene SKC1. In the invention, the vector conversion rate is high; and transgenic corn obtained by the vector has high salt and alkali resistance.

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