The invention relates to a making process of a starch toothpick. The technical scheme comprises the following steps: cleaning corns, sweet potatoes, potatoes or beans and crashing; adding water of one time, stirring, filtering, fermenting, and making into amylase; then taking crashed raw material, adding water of two times, stirring, filtering and fermenting filtrate at 20-25DEG C for 6-8 days, thus obtaining leavening; adding the amylase according to the weight proportion of 100: 1-2, stirring, precipitating, drying, and obtaining starch; taking 15 parts of starch and 2-3 parts of water and mixing well, heating for 5-8 minutes at 100DEG C, then adding 100 parts of the starch, stirring, extruding starch strip semi-finished products by an extruder and then cooling; and then cutting the starch strip with the length of 60-70cm, heating for 5-8 minutes at 100DEG C, taking out and drying and making into the starch toothpick in a round stick machine. The process is simple and the manufactured product has good flexibility.