The invention provides a bacillus amyloliquefacien BZ6-1 strain for producing a biosurfactant and application thereof in oil sludge elution. In the invention, a separated biosurfactant produced by microorganisms is used for treating sludge containing oil so as to achieve the purposes of recovering crude oil deposited in the sludge and reducing oil sludge damages. The strain is preserved in a preservation unit appointed by the State Intellectual Property Office (SIPO) on January 22, 2009, the name of the preservation unit is China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.2892. When the biosurfactant extracted from microorganisms is used for eluting the sludge containing the oil, the average recovery rate of the oil is 87.7 percent, and the content of residual oil in lower sludge is 4.1 percent; and while water is used for contrast treatment, the average recovery rate of the oil is 13.0 percent, and the content of the oil in the lower sludge is 80.6 percent. In addition, the color of the oil sludge treated by the biosurfactant obviously becomes white.