The invention discloses a roof with rectangular plane, super-long span and prestressed space tube truss structure and a manufacturing method. In the invention, the structures of the conventional shuttle shape space tube trusses are taken as the basic frames; support bars for supporting steel cables are respectively fixed at the two ends of each shuttle shape space tube truss; the structure height in the center of each shuttle shape space tube truss is set to be 1/18-1/16 of the span length of the shuttle shape space tube truss; two steel cable turning supports are symmetrically fixed on the shuttle shape space tube truss, close to a lower chord of the shuttle shape space tube truss; the steel cables are respectively supported on the two support bars after passing through the two steel cable turning supports, so that the steel cables between the support bars and the steel cable turning supports are formed into oblique-pulling steel cables and prestressed steel cables with tension, thus ensuring the two steel cable turning support points to generate two elastic reacting forces which act down and up and are opposite to the load action directions so as to compensate insufficient rigidity caused by reduction of the structure height. The invention is suitable for the roof with the ratio of building long side to short side (L1/L2) being not less than1.5 and span length L2 being not less than 90m and not more than 150m. The invention has the characteristics of low structure height, smaller internal force, less steel consumption, low engineering cost, etc.