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发明公开
CN101948794A 产植物类黄酮合成相关酶的工程乳酸菌及其构建和应用 无效 - 驳回

产植物类黄酮合成相关酶的工程乳酸菌及其构建和应用
摘要：
本发明公开了属于基因工程改造乳酸菌领域的产植物类黄酮合成相关酶的工程乳酸菌及其构建和应用。该工程乳酸菌是将完全来自于我国本土栽培大豆(Glycine max(L.)Merr.)的类黄酮合成途径相关酶基因PAL、4CL、CHS和CHI连接成多顺反子结构PAL-4CL-CHS-CHI，构建到经改造的乳酸菌表达载体pMG26e上，形成重组载体pMG26e-4GS，再将pMG26e-4GS电击转化到乳酸菌中，得到产植物类黄酮合成相关酶的基因工程乳酸菌。该重组菌保藏号为CGMCC No.4086，较原始乳酸菌具有更加丰富的生理功能，可直接应用于食品领域，本发明所得到的重组菌株经发酵，类黄酮产量在mg/l水平，高于同类重组大肠杆菌和重组酵母菌发酵的水平。
摘要（英）：

The invention discloses engineering lactobacilli for producing plant flavonoid to synthesize related enzymes, construction and application thereof, belonging to the field of lactobacillus reformation of genetic engineering. The engineering lactobacilli is produced by the steps of: synthesizing flavonoid completely from Chinese locally planted soybeans (Glycine max(L.)Merr.) into related enzyme genes PAL, 4CL, CHS and CHI which are connected into a polycistron structure PLA-4CL-CHS-CHI; then constructing the polycistron structure onto a reformed lactobacillus expression vector pMG26e to form a recombinant vector pMG26e-4GS; and then electrically shocking and converting the pMG26e-4GS into lactobacilli to obtain the engineering lactobacilli for producing plant flavonoid to synthesize related enzymes. The preservation number of the recombinant lactobacilli is CGMCC (China General Microbiological Culture Collection Center) No.4086, and the engineering lactobacilli has more abundant physiological functions compared with original lactobacilli and can be applied to the field of food. Through fermentation, the flavonoid output of the obtained recombinant strain is in the level of mg/l, which is higher than the fermentation levels of like recombinant escherichia coli and recombinant yeast.

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