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发明公开
CN101948828A 一种微量动物细胞基因组DNA模板制备液及相应的DNA模板制备方法 失效 - 权利终止

一种微量动物细胞基因组DNA模板制备液及相应的DNA模板制备方法
摘要：
本发明属于分子生物学领域，公开了一种微量动物细胞基因组DNA模板制备液及相应的DNA模板制备方法。微量动物细胞基因组DNA模板制备液由A液和B液以1:3-10的体积比混合制得，A液为Tris、(NH4)2SO4、MgCl2的混合溶液，三种试剂终浓度分别为0.1-2.0M；所述的B液为Triton和β-琉基乙醇的混合溶液，二种试剂终浓度分别为0.1-2.0%。本发明微量动物细胞基因组DNA模板制备液成本较之现有的基因组DNA提取所需试剂的提取成本大幅降低；由于模板制备过程简单，所制备的基因组DNA模板完整，没有耗损，可确保细胞基因组DNA模板制备的一次性成功。
摘要（英）：

The invention belongs to the field of molecular biology and discloses trace animal cell genomic DNA template preparation solution and a corresponding DNA template preparation method. The trace animal cell genomic DNA template preparation solution is prepared by mixing solution A and solution B in a volume ratio of 1:3-10, wherein the solution A is the mixed solution of Tris, (NH4)2SO4 and MgCl2, and the final concentration of the Tris, the (NH4)2SO4 and the MgCl2 is 0.1 to 2.0M respectively; and the solution B is the mixed solution of Triton and beta-mercaptoethanol, and the final concentration of the Triton and the beta-mercaptoethanol is 0.1 to 2.0 percent respectively. Compared with the cost of the conventional reagent required for extracting genomic DNA, the cost of the trace animal cell genomic DNA template preparation solution is greatly reduced; and as the template preparation process is simple, the prepared genomic DNA template is complete, waste is avoided, and the cell genomic DNA template can be successfully prepared at one time.

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