The invention relates to a preparation method of ZnO-doped PET ultrafine non-woven fabric, which comprises the following steps: (1) mixing zinc salt and surfactant according to the molar ratio of 1:2, dissolving the mixture into an organic solvent, and heating and refluxing for 30min at 80 DEG C; (2) adding deionized water and alkaline agent according to the volume ratio of 1:1, magnetically stirring for 1h at the room temperature, centrifuging, and then, drying in vacuum at the room temperature to obtain nano-ZnO powder; and (3) preparing a hexafluoroisopropanol solution of PET and a toluene solution of nano-ZnO, preparing the two solutions into a ZnO/PET spinning solution according to the volume ratio of 9:1, putting the ZnO/PET spinning solution into an electrostatic spinning device for spinning, and drying the obtained product in vacuum for 24h at the normal temperature. The invention has the advantages of simple processes, easy acquisition and low cost of raw materials, low equipment requirements, low energy consumption and convenient popularization; and the obtained non-woven fabric has excellent anti-ultraviolet performance and does not have obvious defects on the surface.