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发明授权
CN101962690B 检测H1和H3亚型流感病毒的核苷酸序列和试剂盒 失效 - 权利终止

检测H1和H3亚型流感病毒的核苷酸序列和试剂盒
摘要：
本发明公开了一组检测H1和H3亚型流感病毒的核苷酸序列和试剂盒。该检测H1和H3亚型流感病毒的核苷酸序列如序列表SEQ ID NO：1至SEQ ID NO：6，其中序列SEQ ID NO：1和SEQ ID NO：2分别为检测H1亚型流感病毒HA基因的正义引物和反义引物，序列SEQ ID NO：3为检测H1亚型流感病毒HA基因的LNA修饰的荧光探针，序列SEQ ID NO：4和SEQ ID NO：5分别为检测H3亚型流感病毒HA基因的正义引物和反义引物，序列SEQ ID NO：6为检测H3亚型流感病毒HA基因的荧光探针。本发明进一步提供了含有上述引物和探针的检测H1和H3亚型流感病毒的试剂盒。本发明所述的试剂盒具有快速、灵敏、特异、通用性好、重复性好及不易污染的特点。
摘要（英）：

The invention provides a group of nucleotide sequences and a kit for testing H1 and H3 subtypes of influenza viruses. The nucleotide sequences for testing H1 and H3 subtypes of influenza viruses are shown by the sequence table from SEQ ID NO:1 to SEQ ID NO:6, wherein the SEQ ID NO:1 and the SEQ ID NO:2 are respectively a sense primer and an antisense primer for testing the HA gene of H1 subtype of influenza viruses, the SEQ ID NO:3 is an LNA modified fluorescent probe for testing the HA gene of H1 subtype of influenza viruses, the SEQ ID NO:4 and the SEQ ID NO:5 are respectively a sense primer and an antisense primer for testing the HA gene of H3 subtype of influenza viruses, and the SEQ ID NO:6 is a fluorescent probe for testing the HA gene of H3 subtype of influenza viruses. The invention also provides a kit testing H1 and H3 subtypes of influenza viruses, which contains the primers and the probes. The kit has the advantages of quickness, high sensitivity, particularity, commonality and repeativeness, and is difficult to be polluted.

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