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发明公开
CN101962692A 针对HA基因检测甲型H1N1流感病毒2009变异株的核苷酸序列和试剂盒 失效 - 权利终止

针对HA基因检测甲型H1N1流感病毒2009变异株的核苷酸序列和试剂盒
摘要：
本发明公开了一组针对HA基因检测甲型H1N1流感病毒2009变异株的核苷酸序列和试剂盒。该H1N1流感病毒2009变异株的核苷酸序列如序列表SEQ ID NO：1至SEQ ID NO：3，其中序列SEQ ID NO：1和SEQ ID NO：2分别为检测H1N1流感病毒2009变异株HA基因的正义引物和反义引物，序列SEQ ID NO：3为检测H1N1流感病毒2009变异株HA基因的LNA修饰的荧光探针。本发明进一步公开了含有上述引物和探针的检测H1N1流感病毒2009变异株的试剂盒。本发明所述的试剂盒具有快速、灵敏、特异、通用性好、重复性好及不易污染的特点。
摘要（英）：

The invention discloses a group of nucleotide sequences and a kit for detecting H1N1 influenza A virus 2009 variants aiming at HA genes. The nucleotide sequences of the H1N1 influenza virus 2009 variants are expressed as sequence tables SEQ ID No: 1 to SEQ ID No: 3, wherein the sequences SEQ ID No: 1 and SEQ ID No: 2 are a positive primer and a negative primer for detecting the HA genes of the H1N1 influenza virus 2009 variants respectively, and the SEQ ID No: 3 is a fluorescent probe for detecting the LNA modification of the HA genes of the H1N1 influenza virus 2009 variants. The invention further discloses the kit containing the primers and the probe for detecting the H1N1 influenza virus 2009 variants. The kit has the characteristics of quickness, sensitivity, specificity, good generality, good repeatability and difficult pollution.

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