The invention provides a health-care food composition with an anti-fatigue or/and immunity-improving function. The composition is a preparation prepared from rhodiola, hawthorn, Chinese yam, Chinese date, wolfberry, houttuynia cordata, honeysuckle flower, propolis, vitamin C and fructooligosaccharides which serve as raw materials. The invention also provides a preparation method and application of the composition. In the composition, traditional Chinese medicines and a vitamin C antioxidant are organically combined to comprehensively adjust the immunity of human bodies, comprehensively improve the immunity of human body cells and body fluids by helping digestion and promoting digestion, tonifying middle-Jiao and Qi, and nourishing liver, spleen and kidney, increase the excretion of toxic substances and play a role in resisting fatigue and adjusting immunity of human bodies by clearing away heat and toxic materials, clearing away oxygen radicals from human bodies and reducing the generation of toxic substances in the human bodies at the same time. The compatibility of the raw materials fulfils the aim of synergism and provides a new choice for functional foods.