The invention discloses a method for extracting a hoof peptide. Fresh hoof of a healthy pig is used as a raw material in the method; and the hoof peptide is obtained by cleaning, drying, crushing, water distribution, heating and cooking, enzymolysis, enzyme inactivation, decolorization, membrane separation and drying of the obtained filtrate. The method has the advantages of short production period, low cost and high yield (35 percent), and does not produce any toxic or harmful substance; and the obtained product has high safety and can be widely applied in the fields of food, medicaments, health-care products and the like.