The invention relates to a natural nail polish cleaning agent which comprises the following components in parts by weight: 10-20 parts of limonene, 5-15 parts of citral, 0.2-1 part of citric acid and 70-85 parts of triacetin, and the natural nail polish cleaning agent is prepared by stirring uniformly at the speed of 50-70 turns/min under the condition that the temperature is 15 DEG C-25 DEG C. The natural nail polish cleaning agent has stronger cleaning effect for nail polish, and can avoid causing damages to fingernails and effectively avoid the phenomena of dryness and whiteness of the fingernails after cleaning the fingernails with the ordinary nail polish cleaning agent. The nail polish cleaning agent is safe and environment-friendly, and has simple process, simple and convenient useand low cost.