The invention relates to a preparation method of a medical rubber strip, which is preparation method of sterilizing and pest-resistant medical rubber strip containing natural eucalyptus leaf extract. The substrate carrier material of the above medical rubber strip is made of raw material containing eucalyptus leaf extract powders, which enable the medical rubber strip to be bactericidal and pest-resistant. The invention also has the advantages of simple process, convenient operation, low cost, improved sulphuration rate and decreased consumption of raw materials. The performance of the medical rubber strip in the invention is in line with the national standard.