The invention discloses preparation method for a liquid for reducing a bee population taste substance, which is implemented as the following steps: adding wintersweet flowers or citrus flowers from the first blooming period to the full bloom period in a closed container, compacting the flowers, submerging and immersing the flowers immediately by a white spirit at the alcohol concentration of 38-45 degrees for 3 months in a closed mode, filtering and removing flower slag by an absorbent gauze, adding the immersing liquid in a closed container for storage, adding 1-2 droplets of the above immersing liquid in a hive respectively 2-3 hours before merging bee populations and introducing queen bees, performing conventional operation to achieve the aim, and cleaning double hands by utilizing the liquid to operate when queen bees are seized or released. The liquid prepared by the invention has obvious effects, is free of pollution and convenient, and nearly has no stimulation on bee populations.